纯小分子鸡尾酒疗法促进人类多能干细胞向肝细胞的分化 | Stem Cell Research & Therapy
数字识别码：10.1186/-018-0794-4
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原文作者：Cong Du, Yuan Feng, Qiu, Yan Xu, Mao Pang, Nan Cai, Andy Peng Xiang and Qi Zhang
人类诱导多能干细胞的出现为产生充足的各异的肝细胞带来了巨大希望。尽管之前已有工作通过基于病毒的转录因子和/或在分化过程中添加生长因子等技术在体外成功地从人多能干细胞产生了肝细胞，但病毒转导的安全性问题和细胞因子的高成本阻碍了其下游的应用。最近，由于具有优越的稳定性、安全性、细胞渗透性和成本效益，小分子的使用成为诱导细胞命运转变的一种有力工具。
在发表于Stem Cell & 的 and from human stem cells by pure small- 一文中，来自中山大学附属第三医院的Qi Zhang及其研究团队基于纯小分子鸡尾酒疗法，开发了一种新型高效的人类多能干细胞的肝细胞分化方案。这种方法依靠三步策略在短短的13天内，逐步诱导肝细胞的分化，包括定型内胚层分化、肝脏特化和肝细胞成熟。
图1. 诱导hPSC逐步分化为肝细胞的三阶段。
Cong Du et al.
分化的肝细胞样细胞在形态学上类似于基于生长因子法产生的肝细胞和原代肝细胞。这些细胞不仅在转录和蛋白水平上表达特异性肝脏标志物，还具有主要的肝功能，例如白蛋白产生、糖原储存、细胞色素P450的活性和吲哚菁绿的吸收和释放。
通过这种新颖的纯小分子鸡尾酒方法，可以实现人多能干细胞高效快速的肝细胞分化，这为体外研究人类肝脏发育的分子机制提供了一个经济有效的平台，在未来临床应用方面有巨大的潜力。
摘要：
The of human- stem cells holds great for ample . have in from human stem cells in vitro by viral-based of and/or of the , the issue of viral and high cost of would the . , the use of small has as a tool to cell fate for their , , cell , and cost-.
In the study, we a novel of human stem cells with pure small- . This in a , , , and only 13 days.
The -like cells were to from -based and . These cells not only at the and , but also main liver such as , , P450 , and green and .
and from human stem cells could be by our novel, pure, small- , which a cost- for in vitro of the of human liver and holds for .
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期刊介绍:
Stem Cell & ( , 4.963 - 2-year , 5.382 - 5-year ) is the major forum for into stem cell . An peer- , it high- open with a on basic, and into stem cell and , and . The also , , , and .